Linked Life Data

2392327

Source:http://linkedlifedata.com/resource/pubmed/id/2392327

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1990-10-1
pubmed:abstractText
Epidermal growth factor (EGF) and transforming growth factor alpha (TGF alpha) bind to a common cell surface receptor that mediates their diverse biological activities. NIH3T3 fibroblasts transfected with either full-length EGF precursor (preproEGF) or proTGF alpha cDNA displayed distinct patterns of growth in culture. PreproEGF induced focal transformation, and transfectants grew in a chemically defined medium (CDM) at low cell density in the absence of added EGF. In contrast, TGF alpha failed to cause focal transformation, and transfectants grew in CDM in the absence of added growth factors only when seeded at high cell density. The 53 amino acid EGF portion of the preproEGF translation product was essential for its effects. These results indicate that constitutive expression of preproEGF is sufficient to establish autocrine growth of NIH3T3 expressing low levels of EGF receptors. At high cell density, where paracrine as well as autocrine effects of these growth factors would be evident, TGF alpha transfectants displayed at least as high or higher levels of EGF receptor (EGFR) tyrosine phosphorylation than preproEGF transfectants. Since quantitative levels of ligand expression did not account for differences in their transforming properties, preproEGF must be more efficient than proTGF alpha in binding and/or activating EGF receptors in an autocrine manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1265-70
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Transformation of NIH3T3 fibroblasts by an expression vector for the human epidermal growth factor precursor.
pubmed:affiliation
Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article