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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-9-25
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pubmed:abstractText |
The 5-HT3 hydroxytryptamine receptor from NCB 20 cells was solubilized and the molecular and hydrodynamic properties of the receptor were investigated. The receptor was identified by binding of the radioligand 3-NN'-[3H]dimethyl-8-azabicyclo[3.2.1]octanyl indol-3-yl carboxylate ester [( 3H]Q ICS 205-930) to NCB 20 membranes (Bmax = 1.19 +/- 0.31 pmol/mg of protein; Kd = 0.43 +/- 0.076 nM) and was optimally solubilized with 0.5% deoxycholate. [3H]Q ICS 205-930 labelled one population of sites in solution (Bmax = 1.11 +/- 0.4 pmol/mg of protein; Kd = 0.48 +/- 0.06 nM; n = 4). The characteristics of [3H]Q ICS 205-930 binding were essentially unchanged by solubilization, and competition for [3H]Q ICS 205-930 binding by a series of 5-HT3 agonists and antagonists was consistent with binding to a 5-HT3 receptor site and was similar to that observed for 5-HT3 receptors solubilized from rat brain [McKernan, Quirk, Jackson & Ragan (1990) J. Neurochem. 54, 924-930]. Some physical properties of the solubilized receptor were investigated. The molecular size (Stokes radius) of the [3H]Q ICS 205-930-binding site was measured by gel-exclusion chromatography in a buffer containing 0.2% Lubrol and 0.5 M-NaCl and was determined as 4.81 +/- 0.15 nm (mean +/- S.E.M.; n = 6). Sucrose-density-gradient centrifugation was also performed under the same detergent and salt conditions to determine the partial specific volume (v) of the detergent-receptor site complex. This was found to be 0.794 ml.g-1. Sucrose-density-gradient centrifugation was carried out in both 1H2O and 2H2O to allow correction for detergent binding to the receptor. The Mr of the 5-HT3 receptor under these conditions was calculated as 249,000 +/- 18,000 (n = 3). The size and physical properties of the 5-HT3 receptor are similar to those observed for members of the family of ligand-gated ion channels.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-1150088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-13767412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2303819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2449175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2469177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2472553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2479018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2535707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2566650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2667222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2720311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2849052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2893605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2898961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2901973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-2907045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3244388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3272181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3350057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3352595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3368053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3378579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3409965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3412489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3696238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-3707934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-6286620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-6687403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-7051962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2390056-7380005
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
623-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2390056-Animals,
pubmed-meshheading:2390056-Cell Membrane,
pubmed-meshheading:2390056-Centrifugation, Density Gradient,
pubmed-meshheading:2390056-Chromatography, Gel,
pubmed-meshheading:2390056-Indoles,
pubmed-meshheading:2390056-Molecular Weight,
pubmed-meshheading:2390056-Rats,
pubmed-meshheading:2390056-Receptors, Serotonin,
pubmed-meshheading:2390056-Serotonin Antagonists,
pubmed-meshheading:2390056-Tritium,
pubmed-meshheading:2390056-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Molecular size of the 5-HT3 receptor solubilized from NCB 20 cells.
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pubmed:affiliation |
Merck Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, U.K.
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pubmed:publicationType |
Journal Article
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