Linked Life Data

2389989

Source:http://linkedlifedata.com/resource/pubmed/id/2389989

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-9-26
pubmed:abstractText
Functional ultrastructural changes in human sperm heads were evaluated following semen dilution in cryoprotective medium (with and without seminal plasma) as well as freezing/thawing. Plasma membranes were as much altered by dilution as by freezing/thawing when compared with fresh samples. The most striking effect on acrosome noted during freezing/thawing was a dramatic decline in percentage of intact spermatozoa. Acrosomal changes seemed to be less important in the fractions without seminal plasma. These deleterious effects were very demonstrable using transmission electron microscopy (TEM), rather than conventional staining. TEM is useful in obtaining more detailed information on membrane and acrosome integrity until a specific procedure to evaluate functional/physical integrity can be found. Cryopreservation in the absence of seminal plasma can be used for intrauterine insemination in AIH, AID programs with frozen semen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0148-5016
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Ultrastructural changes in membranes and acrosome of human sperm during cryopreservation.
pubmed:affiliation
Laboratoire de Biologie de la Reproduction, C.H.U. Bretonneau, Tours, France.
pubmed:publicationType
Journal Article, Comparative Study