Linked Life Data

2388232

Source:http://linkedlifedata.com/resource/pubmed/id/2388232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1990-9-26
pubmed:abstractText
Yolk proteins (vitellogenin and vitellin) proved to be excellent marker molecules for separating Anopheles gambiae Giles and An. arabiensis Patton, two morphologically indistinguishable members of the An. gambiae species complex. A rabbit polyclonal antibody directed against An. gambiae yolk proteins was made species-specific by removing immunoglobulins that crossreacted with An. arabiensis by immunoaffinity chromatography. The resultant antibody was 400 times more sensitive to An. gambiae and was employed as the secondary antibody in a modified double antibody "sandwich" ELISA, which also used monoclonal antibodies to anopheline vitellogenin as the primary or coating antibody. This ELISA easily differentiated soluble yolk protein samples from An. gambiae and An. arabiensis. A field study with 628 females of An. gambiae complex collected in western Kenya demonstrated that the ELISA results were 98.4% in agreement with the standard cytotaxonomic method.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2585
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
564-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Differentiation of Anopheles gambiae and An. arabiensis (Diptera: Culicidae) by ELISA using immunoaffinity-purified antibodies to vitellogenin.
pubmed:affiliation
Department of Entomology, University of Maryland, College Park 20742.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't