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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-9-27
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pubmed:abstractText |
The T cell product interleukin 5 (IL-5) has been shown to be a key factor in the development and the maturation of the eosinophilic cell lineage. We report here on the detection of human IL-5 receptors on eosinophilic sublines of the promyelocytic leukemia HL-60. Sodium butyrate, which initiates differentiation to mature eosinophils, also induces the appearance of high affinity (Kd 1-5 X 10(-11) M) IL-5 binding sites on these cells. The receptors are specific for IL-5, since binding of radiolabeled ligand can only be inhibited with homologous or murine IL-5 and not by other cytokines. We further show that the receptors are functional, since IL-5 can stimulate the proliferation of these cells. Affinity crosslinking of surface-bound 125I human IL-5 or 35S mouse IL-5 identified two membrane polypeptides of approximately 60 and approximately 130 kD to which IL-5 is closely associated. The presence of granulocyte/macrophage-colony-stimulating factor or tumor necrosis factor during butyrate induction decreased the expression of IL-5 binding sites compared with control cultures. The identification and characterization of human IL-5 receptors on HL-60 sublines should provide new insight into the role of this cytokine in eosinophil differentiation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2460707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2469765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2473157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2506168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2550928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2670497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2673547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-276884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2784567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2787385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2787531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2826636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-2835420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-288488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3024129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3116143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3184985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3257253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3262707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3284812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3486011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3486243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3500861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3501371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3857114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-3968436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-6167441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-6175532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-6588134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-6602251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-6975347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-7229368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2388031-7425292
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
172
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
683-91
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2388031-Butyric Acid,
pubmed-meshheading:2388031-Butyric Acids,
pubmed-meshheading:2388031-Cell Line,
pubmed-meshheading:2388031-Clone Cells,
pubmed-meshheading:2388031-Eosinophils,
pubmed-meshheading:2388031-Humans,
pubmed-meshheading:2388031-Interleukin-5,
pubmed-meshheading:2388031-Kinetics,
pubmed-meshheading:2388031-Leukemia, Promyelocytic, Acute,
pubmed-meshheading:2388031-Receptors, Immunologic,
pubmed-meshheading:2388031-Receptors, Interleukin,
pubmed-meshheading:2388031-Receptors, Interleukin-5,
pubmed-meshheading:2388031-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Characterization of interleukin 5 receptors on eosinophilic sublines from human promyelocytic leukemia (HL-60) cells.
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pubmed:affiliation |
Roche Research Gent, Ghent, Belgium.
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pubmed:publicationType |
Journal Article
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