Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1990-9-27
pubmed:abstractText
We have analyzed both the total ganglioside composition and the surface accessibility of C3H/HeN B lymphocytes and C3H/HeJ B lymphocytes. Seventeen individual resorcinol-positive moieties were visualized by two-dimensional thin-layer chromatography of the purified gangliosides from both strains. Complete homology between strains was seen in the patterns of total gangliosides purified from the endotoxin-responsive and -hyporesponsive strains, with only minor differences in the relative concentrations of four gangliosides. In comparison, only 12 individual gangliosides were accessible to surface labeling following galactose oxidase treatment in these same strains, suggesting that some gangliosides are masked at the cell surface in both strains. However, labeling of the more polar components was greatly reduced in the endotoxin-hyporesponsive (C3H/HeJ) strain, suggesting that these gangliosides have decreased accessibility to galactose oxidase at the cell surface. Therefore, while the total ganglioside compositions of the two strains were nearly equivalent, there were dramatic differences in ganglioside surface accessibility. These findings indicate that an alteration in membrane structure that is associated with the endotoxin hyporesponsiveness observed in C3H/HeJ B lymphocytes exists.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-1185105, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-1250402, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-14066623, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-181911, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-2456326, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-2456339, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-2494280, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-2642946, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-304463, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-305850, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3131472, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3170565, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3264007, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3486829, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3487572, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3494081, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-351618, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3537127, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3540954, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3838173, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-3905790, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-4016115, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-4355577, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-4877918, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-5534017, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-6159304, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-6246515, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-6296882, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-6609839, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-6752418, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-6805963, http://linkedlifedata.com/resource/pubmed/commentcorrection/2387625-6984063
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2888-94
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Altered B-lymphocyte membrane architecture indicated by ganglioside accessibility in C3H/HeJ mice.
pubmed:affiliation
Department of Internal Medicine, Yale University School of Medicine, West Haven, Connecticut.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.