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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1990-9-20
|
| pubmed:abstractText |
We describe a simple, non-radioactive, semi-automated method for measuring lymphocyte ecto-5'-nucleotidase activity in isolated cell homogenates. The method which uses inosine 5'-monophosphate (IMP) as a substrate was optimized and requires a total of 4 x 10(6) lymphocytes. The reference range obtained (38.7-180.0 nmol/h per mg protein; means = 106.6) agrees with other isotopic methods which use 14C-IMP. The simplicity, sensitivity (lower limit 10, upper limit 400 nmol/h per mL) and precision (at 114 nmol/h per mL, interday 4.2% s.d., n = 20; intraday 5.8%, n = 10) makes this method suitable for routine clinical laboratory use.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0818-9641
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
68 ( Pt 2)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
75-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2384259-5'-Nucleotidase,
pubmed-meshheading:2384259-Adolescent,
pubmed-meshheading:2384259-Adult,
pubmed-meshheading:2384259-Aged,
pubmed-meshheading:2384259-Automation,
pubmed-meshheading:2384259-Child,
pubmed-meshheading:2384259-Humans,
pubmed-meshheading:2384259-Inosine Monophosphate,
pubmed-meshheading:2384259-Lymphocytes,
pubmed-meshheading:2384259-Middle Aged,
pubmed-meshheading:2384259-Reference Values,
pubmed-meshheading:2384259-Reproducibility of Results,
pubmed-meshheading:2384259-Substrate Specificity
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
A semi-automated method for 5'-ectonucleotidase measurement in lymphocytes.
|
| pubmed:affiliation |
Department of Pathology, Mater Misericordiae Public Hospital, South Brisbane, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|