Linked Life Data

2381165

Source:http://linkedlifedata.com/resource/pubmed/id/2381165

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-9-13
pubmed:abstractText
Acute ureteral obstruction results in a large influx of macrophages into the cortex and medulla of the obstructed kidney. We examined supernatants from short-term cultures of acutely obstructed and contralateral control renal cortices to determine whether differential production of a chemoattractant for macrophages could account for the cellular infiltrate observed in the obstructed kidney. Using a microchemotaxis assay, supernatants from obstructed renal cortex did demonstrate significantly more chemotactic activity for rat peritoneal macrophages than did supernatants from the contralateral kidneys. This activity showed a dose-response relationship with macrophage migration, peaked between 4 and 12 hours of obstruction, and declined with longer periods of obstruction. The activity was heat stable. It was retained by an octadecylsilane column, and eluted with methanol. Partitioning of the methanol fraction into aqueous and organic phases demonstrated significant activity in the organic phase. In summary, the acutely obstructed rat kidney releases a previously undescribed chemotactic factor that behaves as a lipid. This factor may account for the mononuclear cell infiltrate observed during obstruction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0023-6837
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-20
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Renal cortical release of a specific macrophage chemoattractant in response to ureteral obstruction.
pubmed:affiliation
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't