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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1990-9-12
|
| pubmed:abstractText |
A sensitive and selective high-performance liquid chromatographic (HPLC) method was developed for the determination of the mucoregulatory drug CO/1408 in plasma and urine. Samples containing an internal standard were prepared for analysis using a simple clean-up procedure based on Extrelut solid-phase extraction and chromatographed using a reversed-phase analytical column. Isocratic elution with a mobile phase consisting of 25 mM phosphate buffer (pH 2.5)-acetonitrile-methanol [85:10:5 or 87:9:4 (v/v) for plasma or urine analysis, respectively] was effected at a flow-rate of 0.8 ml/min. The eluate was monitored with an ultraviolet-visible variable-wavelength detector at 200 nm. The limit of quantification for the assay of CO/1408 was 80 ng/ml for plasma and 1 per 0.1 ml for urine samples. In spite of the high solubility of CO/1408 in water, the recovery from plasma and urine was very good and reproducible. The method was found to be applicable to pharmacokinetic studies of the drug in the rat.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-9673
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
16
|
| pubmed:volume |
507
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
227-34
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading | |
| pubmed:year |
1990
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| pubmed:articleTitle |
High-performance liquid chromatographic determination of the mucoregulatory drug CO/1408 in rat plasma and urine.
|
| pubmed:affiliation |
Department of Biochemistry and Pharmacokinetics, Camillo Corvi SpA, Piacenza, Italy.
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| pubmed:publicationType |
Journal Article
|