rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
15
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pubmed:dateCreated |
1990-9-4
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pubmed:databankReference |
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pubmed:abstractText |
A chloroplast outer envelope membrane protein was cloned and sequenced and from the sequence it was possible to deduce a polypeptide of 6.7 kDa. It has only one membrane-spanning region; the C terminus extends into the cytosol, whereas the N terminus is exposed to the space between the two envelope membranes. The protein was synthesized in an in vitro transcription-translation system to study its routing into isolated chloroplasts. The import studies revealed that the 6.7-kDa protein followed a different and heretofore undescribed translocation pathway in the respect that (i) it does not have a cleavable transit sequence, (ii) it does not require ATP hydrolysis for import, and (iii) protease-sensitive components that are responsible for recognition of precursor proteins destined for the inside of the chloroplasts are not involved in routing the 6.7-kDa polypeptide to the outer chloroplast envelope.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-16593034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-16593735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-16654194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-16661696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-16663686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-2449095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-2473899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-2554316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-2642391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-2708339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-2714257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-2848813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-2974457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-3026475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-3780721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-3882712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-6167439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-6181990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-6350279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-6356359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-6382702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-6415062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-6430703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-6798029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-7108955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377616-881736
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Aug
|
pubmed:issn |
0027-8424
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
87
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
5778-82
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:2377616-Amino Acid Sequence,
pubmed-meshheading:2377616-Base Sequence,
pubmed-meshheading:2377616-Chloroplasts,
pubmed-meshheading:2377616-Intracellular Membranes,
pubmed-meshheading:2377616-Kinetics,
pubmed-meshheading:2377616-Membrane Proteins,
pubmed-meshheading:2377616-Molecular Sequence Data,
pubmed-meshheading:2377616-Plants,
pubmed-meshheading:2377616-Protein Biosynthesis,
pubmed-meshheading:2377616-Protein Conformation,
pubmed-meshheading:2377616-Protein Processing, Post-Translational,
pubmed-meshheading:2377616-Thermodynamics
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pubmed:year |
1990
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pubmed:articleTitle |
Sequence analysis and protein import studies of an outer chloroplast envelope polypeptide.
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pubmed:affiliation |
Botanisches Institut, Universität Munich, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|