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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1990-9-4
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pubmed:databankReference |
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pubmed:abstractText |
A genomic clone encompassing the entire gene for the human pyruvate dehydrogenase beta subunit (PDH beta) has been isolated by screening a leukocyte genomic library with a nick-translated human foreskin fibroblast PDH beta cDNA probe. The 18-kilobase clone was characterized by restriction enzyme analysis, extensive DNA sequencing, and primer-extension analysis. The PDH beta structural gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8. The 5' flanking region of the PDH beta gene contains a "CAAT" consensus promoter sequence but no "TATA" sequence. Primer-extension analysis indicated that the PDH beta gene transcription start site is an adenine residue located 132 bases upstream from the initiation codon in exon 1.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-2537010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-2745444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-2792374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-2845947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-3422424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-4307456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-6791577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-797242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2377599-956154
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5594-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2377599-Amino Acid Sequence,
pubmed-meshheading:2377599-Base Sequence,
pubmed-meshheading:2377599-Chromosome Deletion,
pubmed-meshheading:2377599-Cloning, Molecular,
pubmed-meshheading:2377599-Exons,
pubmed-meshheading:2377599-Genetic Vectors,
pubmed-meshheading:2377599-Genomic Library,
pubmed-meshheading:2377599-Humans,
pubmed-meshheading:2377599-Macromolecular Substances,
pubmed-meshheading:2377599-Molecular Sequence Data,
pubmed-meshheading:2377599-Oligonucleotide Probes,
pubmed-meshheading:2377599-Pyruvate Dehydrogenase Complex,
pubmed-meshheading:2377599-Restriction Mapping
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pubmed:year |
1990
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pubmed:articleTitle |
Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene.
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pubmed:affiliation |
Department of Pathological Biochemistry, Nagasaki University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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