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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5-6
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pubmed:dateCreated |
1990-8-23
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pubmed:databankReference | |
pubmed:abstractText |
The expression of the fourth component of complement (C4) of the mouse can differ 20-fold and is determined by C4-high (C4h) or C4-low (C4l) alleles. To investigate the molecular mechanisms underlying the differences in C4 expression, we compared the transcriptional activity of the C4 genes between high and low C4-producer strains of mice (B10 and FM vs B10.BR) using nuclear transcriptional and chloramphenicol acetyltransferase (CAT) assays. We also compared the level of C4-specific RNA in total and nuclear RNA of the liver. The results revealed no significant difference in transcriptional activity between C4h and C4l genes. However, the steady-state levels of C4 mRNA are ten times lower in C4l strains than in C4h strains, suggesting that the major regulation of C4 plasma levels occurs at the post-transcriptional level.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0093-7711
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
361-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2370081-Animals,
pubmed-meshheading:2370081-Base Sequence,
pubmed-meshheading:2370081-Blotting, Northern,
pubmed-meshheading:2370081-Cell Nucleus,
pubmed-meshheading:2370081-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:2370081-Complement C4,
pubmed-meshheading:2370081-Female,
pubmed-meshheading:2370081-Gene Expression Regulation,
pubmed-meshheading:2370081-Liver,
pubmed-meshheading:2370081-Mice,
pubmed-meshheading:2370081-Mice, Inbred Strains,
pubmed-meshheading:2370081-Molecular Sequence Data,
pubmed-meshheading:2370081-RNA, Messenger,
pubmed-meshheading:2370081-RNA Processing, Post-Transcriptional,
pubmed-meshheading:2370081-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2370081-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Post-transcriptional regulation of complement C4 in low C4-producing strain of mouse.
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pubmed:affiliation |
Department of Immunobiology, Kanazawa University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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