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pubmed:abstractText |
Using periodate oxidation, methylation analysis, the characterization of oligosaccharides obtained by partial acid hydrolysis, p.m.r. spectroscopy, and analytical ultracentrifugation, the structure of the (mildly alkali-treated) Klebsiella serotype 11 capusular polysaccharide has been elucidated. The tetrasaccharide repeating-unit comprises the sequence yields 3)-beta-D-Glcp-(1 yields 3)-beta-D-GlcUAp-(1 yields 3)-alpha-D-Galp-(1 yields with a 4,6-O-(1-carboxyethylidene)-alpha-D-galactosyl residue linked to O-4 of the glucuronic acid residue. The structural basis for some serological cross-reactions of the Klebseilla K11 antigen is discussed, and it is shown that rabbit antisera against the Klebsiella K11 test-strain predominantly contain K agglutinins specific for branch-terminal 4,6-O-(1-carboxyethylidene)-D-galactose.
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