Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1990-8-16
|
| pubmed:abstractText |
Although clinical data support the concept of a graft-versus-leukemia (GVL) effect following allogeneic bone marrow transplantation (BMT), there are few data to support a similar GVL activity following syngeneic BMT in man. To identify cells with a potential antileukemic activity post-BMT, we monitored the immunological reconstitution in a patient with chronic phase chronic myeloid leukemia (CML) who received a syngeneic BMT from his identical twin brother. Peripheral blood mononuclear cells (PBMC) from the donor prior to the transplant and from the recipient posttransplant were cultured with recombinant interleukin-2 to generate lymphokine activated killer (LAK) cells. LAK cells from both sources lysed the cell line target cells K562 and LCL and also recipient and allogeneic CML target cells in a 51Cr release cytotoxicity assay. Donor-derived LAK cells did not kill normal donor marrow. LAK cells had similar effects on granulocyte-macrophage progenitor cells (CFU-GM): LAK cells from both donor pre-BMT and recipient post-BMT inhibited the proliferation of CFU-GM from the patient's CML cells, but again donor LAK cells did not inhibit the colony growth of normal donor marrow. These results suggest that a syngeneic GVL effect is inducible following BMT in man and that this activity may be truly antileukemic and spare normal marrow progenitors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0887-6924
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
287-91
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2366584-Adult,
pubmed-meshheading:2366584-Antigens, Neoplasm,
pubmed-meshheading:2366584-Antigens, Surface,
pubmed-meshheading:2366584-Bone Marrow Transplantation,
pubmed-meshheading:2366584-Colony-Forming Units Assay,
pubmed-meshheading:2366584-Graft vs Host Reaction,
pubmed-meshheading:2366584-Granulocytes,
pubmed-meshheading:2366584-Humans,
pubmed-meshheading:2366584-Interleukin-2,
pubmed-meshheading:2366584-Killer Cells, Lymphokine-Activated,
pubmed-meshheading:2366584-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:2366584-Leukocytes, Mononuclear,
pubmed-meshheading:2366584-Macrophages,
pubmed-meshheading:2366584-Male,
pubmed-meshheading:2366584-Phenotype,
pubmed-meshheading:2366584-Transplantation, Isogeneic
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Induction of a syngeneic graft-versus-leukemia effect following bone marrow transplantation for chronic myeloid leukemia.
|
| pubmed:affiliation |
MRC/LRF Leukaemia Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
|
| pubmed:publicationType |
Journal Article
|