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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
1978-3-10
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pubmed:abstractText |
Aqueous biphasic partitioning of Salmonella typhimurium S and R bacteria in a system containing 6.2 per cent (w/w) dextran 500 and 4.4 per cent (w/w) poly(ethyleneglycol) 6000 (PEG) was similar to the partition of the corresponding surface lipopolysaccharide (LPS). Further partition analysis with charged PEG showed that S bacteria and their LPS exposed very little charge, whereas R bacteria and their LPS showed a conspicuous negative charge at neutral pH. Free zone electrophoresis also indicated that the S bacteria have a much lower surface charge density than the R bacteria and accordingly a different surface structure. Thus, the physico-chemical properties of the bacterial surface seem to be determined to a great extent by the characteristics of the cell surface LPS.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
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pubmed:issn |
0105-0656
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
85B
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
334-40
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pubmed:dateRevised |
2009-6-4
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pubmed:meshHeading |
pubmed-meshheading:23649-Cell Wall,
pubmed-meshheading:23649-Counterimmunoelectrophoresis,
pubmed-meshheading:23649-Dextrans,
pubmed-meshheading:23649-Hydrogen-Ion Concentration,
pubmed-meshheading:23649-Immunoelectrophoresis, Two-Dimensional,
pubmed-meshheading:23649-Lipopolysaccharides,
pubmed-meshheading:23649-Polyethylene Glycols,
pubmed-meshheading:23649-Polysaccharides, Bacterial,
pubmed-meshheading:23649-Salmonella typhimurium
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pubmed:year |
1977
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pubmed:articleTitle |
Surface-charge characteristics of smooth and rough Salmonella typhimurium bacteria determined by aqueous two-phase partitioning and free zones electrophoresis.
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pubmed:publicationType |
Journal Article
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