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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-8-14
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pubmed:databankReference |
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pubmed:abstractText |
We have purified extrachromosomal circular DNAs from adult mouse spleen cells, and cloned into a phage vector the BamHl fragments hybridizing with C mu and S gamma 1 probes. We obtained 52 S mu+S gamma 1+ clones by screening 1.4 million phage clones derived from spleen cells stimulated with bacterial lipopolysaccharide and interleukin 4. We have identified the breakpoints of six clones that contain S gamma 1 and S mu sequences fused in the 5' to 3' orientation. All these switch recombination sites were assigned to the central repetitive sequences of the S mu and S gamma 1 regions. Since the common S mu-S gamma 1 sequences at the recombination sites are at most 2 bases long, typical homologous recombination cannot account for their joining. These findings provide direct evidence that mu-gamma 1 class switching can occur by the looping out and excision of chromosomal DNA, with formation of a circle.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
143-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2364432-Animals,
pubmed-meshheading:2364432-B-Lymphocytes,
pubmed-meshheading:2364432-Base Sequence,
pubmed-meshheading:2364432-Cloning, Molecular,
pubmed-meshheading:2364432-DNA, Circular,
pubmed-meshheading:2364432-Female,
pubmed-meshheading:2364432-Gene Library,
pubmed-meshheading:2364432-Genes, Immunoglobulin,
pubmed-meshheading:2364432-Genes, Regulator,
pubmed-meshheading:2364432-Genes, Switch,
pubmed-meshheading:2364432-Genetic Vectors,
pubmed-meshheading:2364432-Mice,
pubmed-meshheading:2364432-Mice, Inbred BALB C,
pubmed-meshheading:2364432-Molecular Sequence Data,
pubmed-meshheading:2364432-Nucleic Acid Hybridization,
pubmed-meshheading:2364432-Oligonucleotide Probes,
pubmed-meshheading:2364432-Plasmids,
pubmed-meshheading:2364432-Recombination, Genetic,
pubmed-meshheading:2364432-Restriction Mapping,
pubmed-meshheading:2364432-Spleen
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pubmed:year |
1990
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pubmed:articleTitle |
Circular DNA is excised by immunoglobulin class switch recombination.
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pubmed:affiliation |
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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