Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1975-7-23
pubmed:abstractText
Methylamine (methylammonium ion) entered Saccharomyces cerevisiae X2180-A by means of a specific active transport system. Methylamine uptake was pH dependent (maximum rate between pH 6.0 and 6.5) and temperature dependent (increasing up to 35 C) and required the presence of a fermentable or oxidizable energy source in the growth medium. At 23 C the vmax for methylamine transport was similar 17 nmol/min per mg of cells (dry weight) and the apparent Km was 220 muM. The transport system exhibited maximal activity in ammonia-grown cells and was repressed 60 to 70 percent when glutamine or asparagine was added to the growth medium. There was no significant derepression of the transport system during nitrogen starvation. Ammonia (ammonium ion) was a strong competitive inhibitor of methylamine uptake, whereas other amines inhibited to a much lesser extent. Mutants selected on the basis of their reduced ability to transport methylamine (Mea-R) simultaneously exhibited a decreased ability to transport ammonia.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-4144122, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-4145865, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-4147647, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-4181698, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-4614980, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-5447432, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-5474888, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-5498410, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-5879894, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-5964977, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-5964978, http://linkedlifedata.com/resource/pubmed/commentcorrection/236281-6048820
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
502-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Methylamine and ammonia transport in Saccharomyces cerevisiae.
pubmed:publicationType
Journal Article