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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1990-8-6
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pubmed:abstractText |
To measure ion activity of intracellular calcium, (Ca)i, we manufactured a Ca2(+)-selective microelectrode based on a neutral carrier, ETH-1001, with or without polyvinylchloride column (PVC-resin). Before and after cell impalements (n = 7), PVC-resin Ca2(+)-selective microelectrodes exhibited Nernstian slopes in the range of p(Ca) from 3 to 7 (the mean slope constant: 29.1 and 29.4 mV/p(Ca), respectively) and sub-Nernstian slopes between p(Ca) = 7 and 8 (21.6 and 21.1 mV/p(Ca]. Individual detection limits of PVC-resin microelectrodes averaged p(Ca) = 8.5 before and 8.3 after cell impalements. In contrast, the non-PVC-resin microelectrode exhibited a poor response between p(Ca) = 7 and 8 especially after cell impalements. Normal values obtained with the PVC-resin microelectrode for (Ca)i of proximal tubule cells and resting sartorius muscle fibers in the bullfrog averaged 18 +/- 2 (n = 10) and 12 +/- 2 (n = 7) nM (mean +/- S.E., number of observations), respectively. We conclude that 1) the PVC-resin microelectrode is a useful tool for measuring intracellular ion activities, and 2) the values of intracellular Ca2+ activity of the proximal tubule and skeletal muscle fibers measured with this microelectrode are in a lower range among the various values reported before with the other methods.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0021-521X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-95
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pubmed:dateRevised |
2007-3-21
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pubmed:meshHeading |
pubmed-meshheading:2362385-Animals,
pubmed-meshheading:2362385-Calcium,
pubmed-meshheading:2362385-Intracellular Fluid,
pubmed-meshheading:2362385-Ionomycin,
pubmed-meshheading:2362385-Kidney Tubules, Proximal,
pubmed-meshheading:2362385-Microelectrodes,
pubmed-meshheading:2362385-Muscles,
pubmed-meshheading:2362385-Phenylephrine,
pubmed-meshheading:2362385-Polyvinyl Chloride,
pubmed-meshheading:2362385-Rana catesbeiana
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pubmed:year |
1990
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pubmed:articleTitle |
Intracellular calcium measurements with PVC-resin Ca-selective microelectrodes in frog proximal tubules and sartorius muscle fibers.
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pubmed:affiliation |
Department of Physiology, Osaka Medical College, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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