Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1990-8-1
pubmed:databankReference
pubmed:abstractText
The first fully repressible gene in fission yeast is described. In minimal medium it is highly transcribed producing a mRNA which is 50-100 times more abundant than the cyc1 mRNA. By contrast, in minimal medium supplemented with thiamine at a concentration of 0.5 microM or greater the transcript is undetectable. The gene has been called nmt1 (for no message in thiamine). The 5' and 3' ends of the transcript have been mapped and indicate an unspliced mRNA of 1.3 kilobases with no evidence of heterogeneity at either end. The single major open reading frame encodes a protein of 39 kDa. The gene product is most likely involved in thiamine biosynthesis and consistent with this is the observation that the nmt1::ura4 disruption strain is a thiamine auxotroph. The kinetics of transcriptional repression and induction have been studied. Addition of thiamine to log phase cells growing minimal medium results in complete disappearance of the nmt1 message within 3 h. Removal of thiamine from the medium produces the first detectable message after 10 h and maximal steady-state levels after 16 h. Nuclear "run on" experiments demonstrate that control is exerted at the level of transcription initiation. The nmt1 promotor has been subcloned, and thiamine-mediated transcriptional control has been transferred to the bacterial reporter gene chloramphenicol acetyltransferase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10857-64
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2358444-Amino Acid Sequence, pubmed-meshheading:2358444-Base Sequence, pubmed-meshheading:2358444-Cloning, Molecular, pubmed-meshheading:2358444-DNA, Fungal, pubmed-meshheading:2358444-DNA Probes, pubmed-meshheading:2358444-Gene Expression Regulation, Fungal, pubmed-meshheading:2358444-Genes, Fungal, pubmed-meshheading:2358444-Kinetics, pubmed-meshheading:2358444-Molecular Sequence Data, pubmed-meshheading:2358444-Nucleic Acid Hybridization, pubmed-meshheading:2358444-Promoter Regions, Genetic, pubmed-meshheading:2358444-RNA, Fungal, pubmed-meshheading:2358444-RNA, Messenger, pubmed-meshheading:2358444-RNA Splicing, pubmed-meshheading:2358444-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:2358444-Restriction Mapping, pubmed-meshheading:2358444-Saccharomycetales, pubmed-meshheading:2358444-Schizosaccharomyces, pubmed-meshheading:2358444-Thiamine, pubmed-meshheading:2358444-Transcription, Genetic
pubmed:year
1990
pubmed:articleTitle
nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.
pubmed:affiliation
Sclavo Research Centre, Siena, Italy.
pubmed:publicationType
Journal Article