Linked Life Data

2357555

Source:http://linkedlifedata.com/resource/pubmed/id/2357555

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1990-8-1
pubmed:abstractText
Peripheral administration of 5-hydroxytryptophan (5-HTP) to rats causes 'wet dog' shakes and a parallel elevation of brain serotonin (5-HT) levels. The increase in 5-HT concentration does not, however, correlate with the endogenous 5-HT innervation raising the possibility that some 5-HTP is decarboxylated in non-serotonergic cells. In the present study we used in vivo voltammetry to establish whether 5-HTP treatment led to formation of 5-HT as a 'false transmitter' in striatal dopamine (DA) neurons. Fast cyclic voltammetry at carbon fibre microelectrodes (CFMs) was used to monitor striatal monoamine release following electrical stimulation of the median forebrain bundle (MFB). In the absence of any pretreatment DA was the sole compound released by stimulation. However, when DA release was abolished with alpha-methyl-p-tyrosine (AMPT), 5-HTP administration (after peripheral decarboxylase inhibition) caused a dose-dependent release of 5-HT, confirmed by the voltammetric characteristics. Central decarboxylase inhibition prevented release indicating that 5-HTP itself was not released. By monitoring reduction peaks it was possible to record DA and 5-HT release simultaneously at a single CFM. While DA and 5-HT oxidised at the same potential their reduction peaks were separated by approximately 450 mV. It was shown, using this means, that 5-HT was still detectable even when DA release was not abolished by AMPT. DA and 5-HT release showed a significant positive correlation suggesting that they were released from the same nerves. We conclude that, after 5-HTP treatment, 5-HT can be released as a false transmitter from striatal DA neurones.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
515
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-80
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Striatal dopamine terminals release serotonin after 5-HTP pretreatment: in vivo voltammetric data.
pubmed:affiliation
Department of Pharmacology, London Hospital Medical College, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't