|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1990-7-24
|
|
pubmed:abstractText |
Human dermal fibroblasts failed to proliferate when cultured in medium containing 0.15 mmol/l (millimolar) Ca2+ (keratinocyte growth medium [KGM]) but did when the external Ca2+ concentration was raised to 1.4 mmol/l. All-trans retinoic acid (retinoic acid) stimulated proliferation in KGM but did not further stimulate growth in Ca2(+)-supplemented KGM. The ability of retinoic acid to stimulate proliferation was inhibited in KGM prepared without Ca2+ or prepared with 0.03 mmol/l Ca2+ and in KGM treated with 1 mmol/l ethylene-glycol-bis-(beta-aminoethyl ether)N,N'-tetra acetic acid. Using 45Ca2+ to measure Ca2+ influx and efflux, it was found that retinoic acid minimally increased Ca2+ uptake into fibroblasts. In contrast, retinoic acid treatment of fibroblasts that had been pre-equilibrated for 1 day with 45Ca2+ inhibited release of intracellular Ca2+ into the extracellular fluid. Retinoic acid also stimulated 35S-methionine incorporation into trichloroacetic acid-precipitable material but in contrast to its effect on proliferation, stimulation of 35S-methionine incorporation occurred in both high-Ca2+ and low-Ca2+ medium. These data indicate that retinoic acid stimulation of proliferation, but not protein synthesis, is dependent on the concentration of Ca2+ in the extracellular environment.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-172436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-195967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-2476509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-2543505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-2708450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-2730659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-2826265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-2918036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-2930546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-2961363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-3102480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-3336176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-3484478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-357215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-3771852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-3771853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-3838803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-3918272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-4060071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-4471173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-6153576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-6256187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-6607257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-6610677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-6723309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-6799829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-6939940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-6945067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2356860-7060138
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0002-9440
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
136
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1275-81
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2356860-Calcium,
pubmed-meshheading:2356860-Cell Division,
pubmed-meshheading:2356860-Cells, Cultured,
pubmed-meshheading:2356860-Culture Media,
pubmed-meshheading:2356860-Dose-Response Relationship, Drug,
pubmed-meshheading:2356860-Fibroblasts,
pubmed-meshheading:2356860-Humans,
pubmed-meshheading:2356860-Keratinocytes,
pubmed-meshheading:2356860-Male,
pubmed-meshheading:2356860-Methionine,
pubmed-meshheading:2356860-Sulfur Radioisotopes,
pubmed-meshheading:2356860-Tretinoin
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Retinoic acid stimulation of human dermal fibroblast proliferation is dependent on suboptimal extracellular Ca2+ concentration.
|
|
pubmed:affiliation |
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
