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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1990-7-18
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pubmed:abstractText |
Glycoprotein V (GPV) is a membrane-associated, 82 Kd platelet glycoprotein that is hydrolyzed during thrombin activation to yield 69 Kd fragment. We have developed a rapid and simple method for isolation of the protein from platelet extracts using a combination of gel permeation, anion-exchange, and lectin affinity chromatography. The partial amino acid sequence was determined by analysis of peptides generated by digestion of the S-carboxyamido-methylated protein with Achromobacter protease I or cyanogen bromide. The sequence shows a remarkable periodicity of leucine residues, which is homologous to the consensus sequence of a highly diversified protein super-family with a common repetitive module. Thrombin cleavage site was determined to be located at the C-terminal region of GPV by analysis of the products separated by sizing and reversed-phase high performance liquid chromatography. By lectin blot analysis, the existence of mucin-type carbohydrate chains was indicated, as well as the existence of asparagine-linked carbohydrate chains shown by the amino acid sequence analysis. From these data, we report a structural model of GPV that is analogous to glycoprotein Ib.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
|
pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
2349-56
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2350580-Amino Acid Sequence,
pubmed-meshheading:2350580-Amino Acids,
pubmed-meshheading:2350580-Blood Platelets,
pubmed-meshheading:2350580-Humans,
pubmed-meshheading:2350580-Isoelectric Point,
pubmed-meshheading:2350580-Molecular Sequence Data,
pubmed-meshheading:2350580-Molecular Weight,
pubmed-meshheading:2350580-Peptide Fragments,
pubmed-meshheading:2350580-Platelet Membrane Glycoproteins
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pubmed:year |
1990
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pubmed:articleTitle |
Rapid purification and characterization of human platelet glycoprotein V: the amino acid sequence contains leucine-rich repetitive modules as in glycoprotein Ib.
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pubmed:affiliation |
Department of Internal Medicine, Hiroshima University, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|