Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1990-7-12
pubmed:abstractText
Anti-phospholipid (aPL) antibodies that exhibit binding in cardiolipin (CL) ELISA can be purified to greater than 95% purity by sequential phospholipid affinity and ion-exchange chromatography. However, these highly purified aPL antibodies do not bind to the CL antigen when assayed by a modified CL ELISA in which the blocking agent does not contain bovine serum, nor do they bind to phospholipid affinity columns. Binding to the phospholipid antigen will only occur if normal human plasma, human serum, or bovine serum is present, suggesting that the binding of aPL antibodies to CL requires the presence of a plasma/serum cofactor. Using sequential phospholipid affinity, gel-filtration, and ion-exchange chromatography, we have purified this cofactor to homogeneity and shown that the binding of aPL antibodies to CL requires the presence of this cofactor in a dose-dependent manner. N-terminal region sequence analysis of the molecule has identified the cofactor as beta 2-glycoprotein I (beta 2GPI) (apolipoprotein H), a plasma protein known to bind to anionic phospholipids. These findings indicate that the presence of beta 2GPI is an absolute requirement for antibody-phospholipid interaction, suggesting that bound beta 2GPI forms the antigen to which aPL antibodies are directed. Recent evidence indicates that beta 2GPI exerts multiple inhibitory effects on the coagulation pathway and platelet aggregation. Interference with the function of beta 2GPI by aPL antibodies could explain the thrombotic diathesis seen in association with these antibodies.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-222615, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-2514785, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-3148207, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-3148208, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-3768409, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-3813670, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-3827975, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-4052628, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-534652, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-6139567, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-629377, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-6294139, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-6427035, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-6587378, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-7389984, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-7417307, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-7444854, http://linkedlifedata.com/resource/pubmed/commentcorrection/2349221-952899
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4120-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Anti-phospholipid antibodies are directed against a complex antigen that includes a lipid-binding inhibitor of coagulation: beta 2-glycoprotein I (apolipoprotein H).
pubmed:affiliation
University of New South Wales, School of Medicine, Saint George Hospital, Kogarah, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't