Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-7-6
pubmed:abstractText
The X protein of hepatitis B virus (HBV) stimulates transcription of a large number of viral enhancers. This protein augments the activity of the HBV enhancer through a specific cis element, termed X responsive element (XRE). Multimers of XRE exhibit enhancer activity which is further stimulated by X. XRE binds multiple cellular transcription factors one of which is the C/EBP. We have constructed the DB gene containing the DNA-binding domain of the C/EBP. This gene efficiently represses the enhancer activity of the XRE by competitive displacement of the XRE-binding factors. Under these conditions, X was found to have only a partially stimulatory effect on transcription, suggesting that the XRE-binding proteins are required for the activity of X. In contrast, an X-DB hybrid protein that binds to the XRE is a strong transcription factor and acts without additional XRE-binding proteins. Furthermore, studies of X mutants revealed that the carboxy-terminus of the protein is required for this activation. These data show that X directly stimulates the cellular transcription machinery, possibly by protein-protein interaction with the XRE-binding factors.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2457861, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2494700, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2521301, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2538828, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2642742, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2667136, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2724417, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2725524, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2786141, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2788226, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2824279, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2826805, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2830620, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2830986, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2830987, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2839300, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2842768, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2843425, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2843426, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2847958, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2850264, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2856254, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2911125, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-2938741, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-3044607, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-3047590, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-3050531, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-3186723, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-3494193, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-3653073, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-3855246, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-3926485, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-4075392, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-6086950, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-6200874, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-6263501, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-6272123, http://linkedlifedata.com/resource/pubmed/commentcorrection/2347309-6960240
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1889-95
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
The X protein of the hepatitis B virus acts as a transcription factor when targeted to its responsive element.
pubmed:affiliation
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't