Linked Life Data

2345680

Source:http://linkedlifedata.com/resource/pubmed/id/2345680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1990-6-29
pubmed:abstractText
The ontogeny of glutamine uptake by jejunal brush border membrane vesicles was studied in suckling and weanling rats and compared with the data obtained from previous studies done on adult rats in our laboratory. Glutamine uptake represented transport into the intravesicular space rather than mere binding into the membrane as evident by osmolality study. The process of glutamine uptake was temperature dependent suggesting a carrier-mediated process with a pH optimum at 7.0. Glutamine uptake was driven by Na+ and K+ gradient in both suckling and weanling rats. Both processes exhibited saturation kinetics and were inhibited by other neutral amino acids suggesting the presence of Na(+)-dependent neutral brush border system and Na(+)-independent (L)-like system. The Vmax of Na(+)-dependent and Na(+)-independent processes were significantly greater in suckling rats with Vmax of 4.9 +/- 0.36 nmol.mg protein-1.7 s-1 compared to weanling rats with Vmax of 2.4 +/- 0.2 nmol.mg protein-1.7 s-1 and adult rats with Vmax of 0.70 nmol.mg protein-1.7 s-1. The greater Vmax in suckling rats is also evident when the kinetic parameters are analyzed by subtracting the sodium-dependent uptake values from the sodium-independent values. Vmax of 1.59 +/- 0.3 and 0.76 +/- 0.01 nmol.mg protein-1. 7 s-1 in suckling and weanling rats, respectively, p less than 0.01. Km values were not different at 2.5 +/- 0.6 and 3.5 +/- 0.6 mM, respectively). The data suggest that the activity and/or the number of transporters are greater during the period of active growth and development.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0031-3998
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
519-24
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Maturational changes in glutamine transport by rat jejunal brush border membrane vesicles.
pubmed:affiliation
Department of Pediatrics, Vanderbilt University Medical School, Nashville, Tennessee 37232.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.