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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-6-28
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pubmed:abstractText |
The synthesis and release of 11-cis-retinoids by primary cultures of human retinal pigment epithelium (RPE) and the transfer of these retinoids to co-incubated human rod outer segments (ROS) were studied. Monolayers of 2-3-week-old cultured RPE incorporate tritiated all-trans-retinol, esterify it to the corresponding retinyl palmitate, form 11-cis-retinol and 11-cis-retinaldehyde and release retinaldehyde into the culture medium. The ratio of 11-cis to all-trans isomers of retinol, retinyl palmitate and retinaldehyde formed in the cells along with retinaldehyde released and incorporated into the ROS progressively increases, indicating a progressive increase in the concentration of 11-cis isomer from the time it is formed in RPE cells until its transfer to ROS. Incorporation of 11-cis-retinaldehyde into the ROS is directly related to the amount of albumin present in the media, suggesting the transfer of retinoids from RPE to photoreceptor to be a protein-mediated process. Events leading to isomerization, esterification, oxidation and release of retinoids by human RPE and incorporation of retinoids into ROS can therefore be examined in vitro.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-1125252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-13549472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-14247650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-2643589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-2727688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-2910871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-3355533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-3372515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-3427115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-3494246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-3500173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-3584132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-4038696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-4418913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-6930647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-7000727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2344357-7190030
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
201-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2344357-Adult,
pubmed-meshheading:2344357-Aged,
pubmed-meshheading:2344357-Biological Transport,
pubmed-meshheading:2344357-Cells, Cultured,
pubmed-meshheading:2344357-Chromatography, High Pressure Liquid,
pubmed-meshheading:2344357-Esterification,
pubmed-meshheading:2344357-Humans,
pubmed-meshheading:2344357-Kinetics,
pubmed-meshheading:2344357-Middle Aged,
pubmed-meshheading:2344357-Oxidation-Reduction,
pubmed-meshheading:2344357-Photoreceptor Cells,
pubmed-meshheading:2344357-Pigment Epithelium of Eye,
pubmed-meshheading:2344357-Retinaldehyde,
pubmed-meshheading:2344357-Retinoids,
pubmed-meshheading:2344357-Rod Cell Outer Segment,
pubmed-meshheading:2344357-Stereoisomerism,
pubmed-meshheading:2344357-Vitamin A
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pubmed:year |
1990
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pubmed:articleTitle |
Synthesis of retinoids by human retinal epithelium and transfer to rod outer segments.
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pubmed:affiliation |
Department of Ophthalmology, Columbia University, New York, NY 10032.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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