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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1990-6-27
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pubmed:abstractText |
The effects of phorbol 12,13-dibutyrate (PDBu). a protein kinase C activator, on the tone and intracellular calcium level (Cai2+) of vascular smooth muscles were simultaneously measured by use of a force-displacement transducer and the fura-2 microscopic fluorometric technique in porcine coronary arteries. Cumulatively applied PDBu produced a slowly developing and concentration-dependent contraction in the concentration range of 10(-8)-10(-6) mol/l. Contractions induced by PDBu were sustained after removal of PDBu. Changes in Cai2+ produced by PDBu were very slight, although Cai2+ was increased at lower concentrations (3 x 10(-8) and 10(-7) mol/l) and decreased at higher concentrations (3 x 10(-7) and 10(-6) mol/l). Verapamil 10(-6) mol/l partially inhibited the contractions throughout the concentration range of PDBu and the increase in Cai2+ induced by lower concentrations of PDBu. When the effects of PDBu were compared with those of the 90 mmol/l KCl medium, the force of contraction induced by a single concentration of 10(-7) mol/l PDBu was about 50% and the increase in Cai2+ was about 10%. Removal of extracellular calcium by 1 mmol/l EGTA decreased Cai2+ by about 20% but vascular tone did not change. PDbu (10(-7) mol/l) produced a small contraction without an increase in Cai2+ in the Ca2+-free medium. Depolarization by the 20 mmol/l KCl medium increased Cai2+ by about 20%, whereas vascular tone did not change.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol 12,13-Dibutyrate,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Verapamil
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0028-1298
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
341
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
251-5
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2342605-Animals,
pubmed-meshheading:2342605-Calcium,
pubmed-meshheading:2342605-Coronary Vessels,
pubmed-meshheading:2342605-Fluorescent Dyes,
pubmed-meshheading:2342605-Muscle, Smooth, Vascular,
pubmed-meshheading:2342605-Muscle Tonus,
pubmed-meshheading:2342605-Phorbol 12,13-Dibutyrate,
pubmed-meshheading:2342605-Potassium Chloride,
pubmed-meshheading:2342605-Protein Kinase C,
pubmed-meshheading:2342605-Swine,
pubmed-meshheading:2342605-Verapamil
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pubmed:year |
1990
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pubmed:articleTitle |
Phorbol 12,13-dibutyrate increases vascular tone but has a dual action on intracellular calcium levels in porcine coronary arteries.
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pubmed:affiliation |
Department of Pharmacology, Tohoku University School of Medicine, Sendai, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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