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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-6-21
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pubmed:abstractText |
Ten oligomannose-type sugar chains (ManGlcNAc2-Man5GlcNAc2) were prepared from various glycoproteins and fluorescence labeled with 2-aminopyridine. The fluorescent pyridylamino (PA)-sugar chains were first separated into five fractions according to their molecular sizes by HPLC on a TSK gel Amide-80 column. Each fraction was then separated into the component PA-sugar chains by reversed-phase HPLC on a Capcell Pak C18 column according to their chemical structures. The method is useful for studying the substrate specificities of alpha-mannosidases with Man5GlcNAc2-PA as a substrate.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2339788-Aminopyridines,
pubmed-meshheading:2339788-Animals,
pubmed-meshheading:2339788-Carbohydrate Sequence,
pubmed-meshheading:2339788-Chickens,
pubmed-meshheading:2339788-Chromatography, High Pressure Liquid,
pubmed-meshheading:2339788-Fabaceae,
pubmed-meshheading:2339788-Mannose,
pubmed-meshheading:2339788-Mannosidases,
pubmed-meshheading:2339788-Molecular Sequence Data,
pubmed-meshheading:2339788-Oligosaccharides,
pubmed-meshheading:2339788-Plants, Medicinal,
pubmed-meshheading:2339788-Quail,
pubmed-meshheading:2339788-Substrate Specificity
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pubmed:year |
1990
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pubmed:articleTitle |
Separation of oligomannose-type sugar chains having one to five mannose residues by high-performance liquid chromatography as their pyridylamino derivatives.
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pubmed:affiliation |
Department of Chemistry, Osaka University College of Science, Japan.
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pubmed:publicationType |
Journal Article
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