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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1993-7-22
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pubmed:abstractText |
Using limiting dilutions of responding cells in mouse mixed leukocyte cultures, we obtained direct estimates of the minimum frequency of precursors of cytotoxic T lymphocytes (CTL.P) for a variety of antigens. Depending on the strain combination, there were as many as 4-15 CTL.P reactive to DBA/2 among 10(4) lymph node cells. Taking into account that only 5-10% of peripheral T lymphocytes have the potential to develop into cytotoxic T lymphocytes (CTLs) (6), this implies that at least 1-2% of all CTL.P are responsive to any given H-2 haplotype difference. Precursors of cytotoxic cells thus have the same high frequency of cells reactive to alloantigens of the major histocompatibility complex as found among proliferating cells in graft-vs.-host reactions and mixed lymphocyte interactions. The frequencies of CTL.P reactive to xenoantigens (rat) or trinitrophenyl-modified self were less than half the frequency of alloreactive CTL.P. A minority of the CTL.P specific for one H-2 haplotype were also reactive to a third party H-2 haplotype, presumably on the basis of recognition of shared determinants. By dilution of sensitized cells from single microcultures, it was shown that a single CTL.P undergoes a minimum of three to four cell divisions and generates at least 8-16 CTLs after antigenic activation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-1083887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-1083888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-1083894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-1084405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-1085418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-1092798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-1092799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-1184965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-127008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-127017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-233898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-235003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-235004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4151339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4151904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4267047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4273650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4277395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4279273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4543318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4545893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4547153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-4625441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-5034145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-52182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-5256217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-53262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-5553067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-5682942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233899-62665
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
145
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
508-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:233899-Animals,
pubmed-meshheading:233899-Cell Division,
pubmed-meshheading:233899-Cells, Cultured,
pubmed-meshheading:233899-Clone Cells,
pubmed-meshheading:233899-Female,
pubmed-meshheading:233899-Hematopoietic Stem Cells,
pubmed-meshheading:233899-Histocompatibility Antigens,
pubmed-meshheading:233899-Leukocyte Count,
pubmed-meshheading:233899-Lymphocyte Activation,
pubmed-meshheading:233899-Male,
pubmed-meshheading:233899-Mice,
pubmed-meshheading:233899-Mice, Inbred Strains,
pubmed-meshheading:233899-T-Lymphocytes, Cytotoxic
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pubmed:year |
1977
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pubmed:articleTitle |
Histocompatibility antigen-activated cytotoxic T lymphocytes. II. Estimates of the frequency and specificity of precursors.
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pubmed:affiliation |
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19174.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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