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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2 Pt 1
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pubmed:dateCreated |
1987-4-21
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pubmed:abstractText |
Electrophoretic data from both sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and acid-urea gels reveal a protein in purified murine mammary tumor virus (MuMTV) which co-migrates with purified chick skeletal muscle actin. 125I-labeling of intact and disrupted virus preparations shows that the actin-like protein is not artifactually adsorbed to the outside of virions during isolation. Quantitative SDS-PAGE and examination of negatively stained preparations show that the actin cannot be accounted for by a contaminating population of virus-free vesicles. The ultrastructure of mammary epithelial cells and of Rous sarcoma virus-transformed chick embryo fibroblasts shows that virus extrusion is associated with filament-containing cellular processes. In particular, MuMTV is released from the ends of long microvilli which contain a bundle of 6-8-nm microfilaments and share other structural features with intestinal microvilli. We suggest that virus nucleoids require an interaction with host cell contractile proteins for their extrusion from the cell.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-1033184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-1095652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-1171267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-1202021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-169380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-174295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-181596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-192451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4140510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4209264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4253329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4254541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4266351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4285211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4361099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4598854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4616399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-4937093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-5076780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233748-808396
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
593-605
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:233748-Actins,
pubmed-meshheading:233748-Animals,
pubmed-meshheading:233748-Chickens,
pubmed-meshheading:233748-Female,
pubmed-meshheading:233748-Lactation,
pubmed-meshheading:233748-Mammary Neoplasms, Experimental,
pubmed-meshheading:233748-Mammary Tumor Virus, Mouse,
pubmed-meshheading:233748-Mice,
pubmed-meshheading:233748-Microscopy, Electron,
pubmed-meshheading:233748-Milk,
pubmed-meshheading:233748-Muscles,
pubmed-meshheading:233748-Pregnancy
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pubmed:year |
1977
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pubmed:articleTitle |
Is there a role for actin in virus budding?
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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