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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1990-6-12
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pubmed:abstractText |
This work describes the molecular properties of the polypeptide core of a human pancreatic mucin antigen isolated from a human pancreatic adenocarcinoma cell line, HPAF. Pancreatic tumor mucin was isolated by a combination of molecular sieve chromatography and CsCl/4 M guanidine-HCl density gradient ultracentrifugation. Trifluoromethane sulfonic acid was used to remove carbohydrate units from purified mucin molecules. A rabbit monospecific polyclonal antibody was generated against pancreatic apomucin and reacted with a Mr greater than 200,000 species. The antibody binding data indicated that the rabbit antiserum raised against pancreatic apomucin cross-reacted with a breast mucin synthetic peptide. Northern blot and immunodot blot analyses of various cell line extracts revealed that a tandem repeat sequence and a similar mRNA were detected in both pancreatic and breast mucin-producing cell lines. These results suggest that pancreatic apomucin and breast apomucin share some similarity in tandem repeated nucleic acid and protein sequences.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2997-3001
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2334903-Adenocarcinoma,
pubmed-meshheading:2334903-Amino Acid Sequence,
pubmed-meshheading:2334903-Amino Acids,
pubmed-meshheading:2334903-Antigens, Neoplasm,
pubmed-meshheading:2334903-Base Sequence,
pubmed-meshheading:2334903-Blotting, Northern,
pubmed-meshheading:2334903-Blotting, Western,
pubmed-meshheading:2334903-Humans,
pubmed-meshheading:2334903-Immunoblotting,
pubmed-meshheading:2334903-Molecular Sequence Data,
pubmed-meshheading:2334903-Mucins,
pubmed-meshheading:2334903-Neoplasm Proteins,
pubmed-meshheading:2334903-Oligonucleotide Probes,
pubmed-meshheading:2334903-Pancreatic Neoplasms,
pubmed-meshheading:2334903-RNA, Messenger,
pubmed-meshheading:2334903-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2334903-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Polypeptide core of a human pancreatic tumor mucin antigen.
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pubmed:affiliation |
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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