rdf:type |
|
lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-5-31
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pubmed:abstractText |
The influence of N-ethylmaleimide (NEM) on contractions due to exogenously applied noradrenaline and bethanechol and on the inhibitory effects of clonidine, of the enkephalin derivative, FK 33-824, and 2-chloroadenosine (2-CLA) on field stimulation-response curves and [3H]noradrenaline [( 3H]NA) release was studied in the isolated mouse vas deferens. Exposure to NEM (60 microM: 10 min) caused a 30% reduction of the maximal contraction due to NA but nearly abolished the response to bethanechol. NEM partially reversed the depression of the pulse width-response curves by clonidine and FK 33-824 but was without effect with 2-CLA. The contractions evoked by stimulation frequencies above 20 Hz were depressed by NEM both in presence and absence of the agonists. NEM diminished the inhibition of the stimulation-evoked release of [3H]NA by the three agonists. The prejunctional effect of NEM was markedly influenced by the stimulation parameters. These findings support the suggestion that the inhibition mediated by alpha 2-adrenoceptors, mu- and P1-receptors in the mouse vas deferens is NEM-sensitive and possibly transmitted by a pertussis toxin-sensitive G-protein.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Chloroadenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Bethanechol Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Clonidine,
http://linkedlifedata.com/resource/pubmed/chemical/D-Ala(2),MePhe(4),Met(0)-ol-enkephal...,
http://linkedlifedata.com/resource/pubmed/chemical/Dioxanes,
http://linkedlifedata.com/resource/pubmed/chemical/Ethylmaleimide,
http://linkedlifedata.com/resource/pubmed/chemical/Idazoxan,
http://linkedlifedata.com/resource/pubmed/chemical/Norepinephrine
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
|
pubmed:issn |
0014-2999
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
20
|
pubmed:volume |
178
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
151-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2328759-2-Chloroadenosine,
pubmed-meshheading:2328759-Animals,
pubmed-meshheading:2328759-Bethanechol Compounds,
pubmed-meshheading:2328759-Calcium Chloride,
pubmed-meshheading:2328759-Clonidine,
pubmed-meshheading:2328759-D-Ala(2),MePhe(4),Met(0)-ol-enkephalin,
pubmed-meshheading:2328759-Dioxanes,
pubmed-meshheading:2328759-Electric Stimulation,
pubmed-meshheading:2328759-Ethylmaleimide,
pubmed-meshheading:2328759-Idazoxan,
pubmed-meshheading:2328759-Male,
pubmed-meshheading:2328759-Mice,
pubmed-meshheading:2328759-Muscle, Smooth,
pubmed-meshheading:2328759-Norepinephrine,
pubmed-meshheading:2328759-Vas Deferens
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pubmed:year |
1990
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pubmed:articleTitle |
Pre- and postjunctional effects of N-ethylmaleimide in the isolated mouse vas deferens.
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pubmed:affiliation |
Department of Pharmacology, Medical University Lübeck, F.R.G.
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pubmed:publicationType |
Journal Article,
In Vitro
|