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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1990-5-24
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pubmed:abstractText |
Bordetella pertussis, the etiological agent of whooping cough, synthesizes a calmodulin-sensitive adenylate cyclase that is suspected to play a major role in the virulence of this bacterium. We show that adenylate cyclase synthesized as a 200-kilodalton protein is the product of the cyaA gene and that various virulent Bordetella species secrete this high-molecular-weight polypeptide without apparent proteolytic processing. When submitted to trypsin digestion, the 200-kilodalton protein was converted to a stable 45- to 50-kilodalton species. This corresponds to the size of the enzyme previously purified from a culture supernatant. The molecular heterogeneity reported for the various identified forms of adenylate cyclase could therefore result in part from proteolytic degradation or molecular aggregation of the major 200-kilodalton form of the enzyme.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-14413273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-182669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2450573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2470405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2540797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2554887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2555185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2859287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2867778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2877614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2877986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2893792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2897067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2901413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2905265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2907365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-2982796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-3891742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-4324651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-6253992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-6287574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-6311749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2323814-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
58
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1195-200
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2323814-Adenylate Cyclase,
pubmed-meshheading:2323814-Amino Acids,
pubmed-meshheading:2323814-Bacterial Proteins,
pubmed-meshheading:2323814-Blotting, Western,
pubmed-meshheading:2323814-Bordetella pertussis,
pubmed-meshheading:2323814-Cloning, Molecular,
pubmed-meshheading:2323814-Genes, Bacterial,
pubmed-meshheading:2323814-Molecular Weight,
pubmed-meshheading:2323814-Peptide Mapping,
pubmed-meshheading:2323814-Restriction Mapping,
pubmed-meshheading:2323814-Trypsin
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pubmed:year |
1990
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pubmed:articleTitle |
Synthesis and secretion of Bordetella pertussis adenylate cyclase as a 200-kilodalton protein.
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pubmed:affiliation |
Unité de Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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