Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1980-6-16
|
| pubmed:abstractText |
RNA and protein of the defective avian acute leukemia virus CMII, which causes myelocytomas in chickens, and of CMII-associated helper virus (CMIIAV) were investigated. The RNA of CMII measured 6 kilobases (kb) and that of CMIIAV measured 8.5 kb. By comparing more than 20 mapped oligonucleotides of CMII RNA with mapped and nonmapped oligonucleotides of acute leukemia viruses MC29 and MH2 and with mapped oligonucleotides of CMIIAV and other nondefective avian tumor viruses, three segments were distinguished in the oligonucleotide map of CMII RNA: (i) a 5' group-specific segment of 1.5 kb which was conserved among CMII, MC29, and MH2 and also homologous with gag-related oligonucleotides of CMIIAV and other helper viruses (hence, group specific); (ii) an internal segment of 2 kb which was conserved specifically among CMII, MC29, and MH2 and whose presence in CMII lends new support to the view that this class of genetic elements is essential for oncogenicity, because it was absent from an otherwise isogenic, nontransforming helper, CMIIAV; and (iii) a 3' group-specific segment of 2.5 kb which shared 13 of 14 oligonucleotides with CMIIAV and included env oligonucleotides of other nondefective viruses of the avian tumor virus group (hence, group specific). This segment and analogous map segments of MC29 and MH2 were not conserved at the level of shared oligonucleotides. CMII-transformed cells contained a nonstructural, gag gene-related protein of 90,000 daltons, distinguished by its size from 110,000-daltom MC29 and 100,000-dalton MH2 counterparts. The gag relatedness and similarity to the 110,000-dalton MC29 counterpart indicated that the 90,000-dalton CMII protein is translated from the 5' and internal segments of CMII RNA. The existence of conserved 5' and internal RNA segments and conserved nonstructural protein products in CMII, MC29, and MH2 indicates that these viruses belong to a related group, termed here the MC29 group. Viruses of the MC29 group differ from one another mainly in their 3' RNA segments and in minor variations of their conserved RNA segments as well as by strain-specific size markers of their gag-related proteins. Because (i) the conserved 5' gag-related and internal RNA segments and their gag-related, nonvirion protein products correlate with the conserved oncogenic spectra of the MC29 group of viruses and because (ii) the internal RNA sequences and nonvirion proteins are not found in nondefective viruses, we propose that the conserved RNA and protein elements are necessary for oncogenicity and probably are the onc gene products of the MC29 group of viruses.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-1102604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-169008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-169037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-170411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-174108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-177972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-199997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-199998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-200913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-200940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-210569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-211711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-214581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-215208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-215788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-215999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-221900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-232456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-4119788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-4135171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-4351610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-4372615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/232172-74287
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-538X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
32
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
208-19
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:232172-Animals,
pubmed-meshheading:232172-Avian leukosis virus,
pubmed-meshheading:232172-Cell Line,
pubmed-meshheading:232172-Defective Viruses,
pubmed-meshheading:232172-Helper Viruses,
pubmed-meshheading:232172-Molecular Weight,
pubmed-meshheading:232172-Nucleic Acid Conformation,
pubmed-meshheading:232172-Oligonucleotides,
pubmed-meshheading:232172-Oligoribonucleotides,
pubmed-meshheading:232172-Protein Conformation,
pubmed-meshheading:232172-Quail,
pubmed-meshheading:232172-RNA, Viral,
pubmed-meshheading:232172-Viral Proteins
|
| pubmed:year |
1979
|
| pubmed:articleTitle |
Oligoribonucleotide map and protein of CMII: detection of conserved and nonconserved genetic elements in avian acute leukemia viruses CMII, MC29, and MH2.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|