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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1990-5-3
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pubmed:databankReference | |
pubmed:abstractText |
Preparations of mRNA isolated from rabbit lung and liver were used in the construction of libraries that were screened for cDNAs encoding the pulmonary or hepatic isozyme of the flavin-containing monooxygenase. The hepatic library was screened with cDNA encoding the flavin-containing monooxygenase expressed in pig liver, and a clone containing a 2.0-kilobase insert was detected and isolated. This cDNA insert encoded a protein of 535 amino acids with a primary structure 87% identical to that of the pig flavin-containing monooxygenase. The pulmonary library was screened with polyclonal antibodies to the flavin-containing monooxygenase expressed in rabbit lung, and a clone containing a 2.6-kilobase insert was detected and isolated. Although the protein encoded by this insert also contained 535 amino acids, its primary sequence was only 56% identical to that of the liver enzyme. The sequences of several peptides obtained by digestion of the purified rabbit pulmonary flavin-containing monooxygenase with trypsin matched exactly with sequences derived from the cDNA structure. Tissue-specific distribution of mRNA for the hepatic and pulmonary isozymes of the flavin-containing monooxygenase was consistent with the distribution of protein, an indication that expression of flavin-containing monooxygenase is controlled at the level of transcription. Analysis of genomic DNA indicates that both the hepatic and pulmonary enzymes may be products of single genes.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Flavin-Adenine Dinucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/NADP,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/dimethylaniline monooxygenase...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
265
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5855-61
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2318837-Amino Acid Sequence,
pubmed-meshheading:2318837-Animals,
pubmed-meshheading:2318837-Base Sequence,
pubmed-meshheading:2318837-Binding Sites,
pubmed-meshheading:2318837-DNA,
pubmed-meshheading:2318837-Flavin-Adenine Dinucleotide,
pubmed-meshheading:2318837-Gene Expression,
pubmed-meshheading:2318837-Isoenzymes,
pubmed-meshheading:2318837-Kidney,
pubmed-meshheading:2318837-Liver,
pubmed-meshheading:2318837-Lung,
pubmed-meshheading:2318837-Molecular Sequence Data,
pubmed-meshheading:2318837-NADP,
pubmed-meshheading:2318837-Nucleic Acid Hybridization,
pubmed-meshheading:2318837-Oxygenases,
pubmed-meshheading:2318837-RNA, Messenger,
pubmed-meshheading:2318837-Rabbits,
pubmed-meshheading:2318837-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2318837-Swine,
pubmed-meshheading:2318837-Tissue Distribution
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pubmed:year |
1990
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pubmed:articleTitle |
The flavin-containing monooxygenase enzymes expressed in rabbit liver and lung are products of related but distinctly different genes.
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pubmed:affiliation |
Laboratory of Cellular and Molecular Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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