Linked Life Data

2313140

Source:http://linkedlifedata.com/resource/pubmed/id/2313140

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-4-24
pubmed:abstractText
The clonal growth of myeloid colonies from peripheral blood was maximal when cultures were established with an initial osmolality of 220 mosmol/kg which increased during incubation as a result of partial drying. When osmolality was stabilized by secondary humidification, the optimum osmolality was 270 mosmol/kg, but growth was always two- to fivefold less than similar cultures established at low osmolality and incubated on an open shelf. Cultures established at 270 mosmol/kg or above were statistically similar whether or not drying was eliminated. Maximum colonies were apparent after 14 days incubation under both conditions; addition of conditioned medium did not alter the pattern of growth. The greater sensitivity of cultures established at 220 mosmol/kg is advantageous when assaying circulating progenitors in pathological conditions where a low number of granulocyte/macrophage colony-forming units is common.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0737-1454
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Stimulation of myeloid colony growth from peripheral blood by medium with an initially low osmolality.
pubmed:affiliation
Department of Haematology, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom.
pubmed:publicationType
Journal Article