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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1990-4-19
|
| pubmed:abstractText |
The ability of the N2 retrovirus to introduce the selectable neo gene into (transform) caprine hematopoietic cells (CHC) was evaluated. Helper-free amphotropic retrovirus producing cells (RPC) were plated at approximately 1.0 x 10(5) cells per 25 cm2 tissue culture flask, cultured to 80% confluence and irradiated (1,500 rads) prior to CHC incubation. The CHC collected from three donor goats were washed and cultured for 24 h in either the RPC or control flasks. Cells were cultured in Dulbecco's Modified Eagles Medium containing 10% fetal calf serum (FCS) (DMEM) and 4 micrograms polybrene/ml. After 48 h of culture in fresh DMEM, cells were recovered and suspended in Iscove's DMEM supplemented with .3% gar, 12% FCS and 400 micrograms geneticin/ml (G418; neomycin) and transferred to 35-mm petri dishes (7.5 x 10(5) cells) for selection of G418 resistant cells. After 17 d of culture, plates were evaluated for total number of colony forming units (CFU, greater than 10 cells). Total number of CFU was greater (P less than .01) in treatment samples (means = 175, SEM = 70) than in control cultures (mean = 0, SEM = 0). The N2 retrovirus appears to be an effective vector for the transformation of CHC and may provide a means to introduce gene(s) into cells of domestic animals.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0021-8812
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
68
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
449-53
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2312432-Animals,
pubmed-meshheading:2312432-Cell Line,
pubmed-meshheading:2312432-Drug Resistance, Microbial,
pubmed-meshheading:2312432-Female,
pubmed-meshheading:2312432-Gene Expression,
pubmed-meshheading:2312432-Genetic Vectors,
pubmed-meshheading:2312432-Goats,
pubmed-meshheading:2312432-Hematopoietic Stem Cells,
pubmed-meshheading:2312432-Retroviridae,
pubmed-meshheading:2312432-Transfection
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Incorporation and expression of a neo gene after transfer to caprine hematopoietic cells using a modified retrovirus.
|
| pubmed:affiliation |
Dept. of Anim. Sci., Louisiana State University, Baton Rouge.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|