Linked Life Data

2308229

Source:http://linkedlifedata.com/resource/pubmed/id/2308229

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-4-10
pubmed:abstractText
A conventional chromogenic limulus test (CCLT, normal plasma value: less than 10 pg/ml) detects both of endotoxin and beta-1,3-glucan, while a chromogenic endotoxin-specific test (EST: less than 3 pg/ml) detects only endotoxin. Two sources of pseudo-positive result in CCLT and/or EST were studied; (1) factor G-activating material found in blood products, which is indicative of fungal infections, and (2) interaction in diazo-coupling process of the tests by antibiotics or sulfonamides. CCLT and EST values obtained from 13 vials of blood products including human albumin, antithrombin III, and antihemophilic globulin were 284.6 +/- 284.6 pg/ml and 20.2 +/- 26.4 pg/ml (mean +/- SD), respectively. High CCLT values accompanied by low EST values indicate factor G-activating material in the blood products rather than endotoxin. Sample blanks of 42 plasma specimens obtained from 19 patients were 23.7 +/- 34.7 pg/ml by CCLT and 19.2 +/- 31.5 pg/ml by EST. All the patients were administered Sulfamethoxazole/Trimethoprim (SMX/TMP), Aztreonam (AZT), Ceftazidime (CAZ), Imipenem/Cilastatin (IPM/CS), or Silver sulfadiazine. SMX/TMP, AZT, CAZ, IPM/CS, and Cefmenoxime were demonstrated to react with the diazo-coupling reagents used in CCLT and EST, and to cause false-positive result in the tests. Thus the investigation of blood product and sample blank is required for the assay of endotoxins or fungal polysaccharides using CCLT and EST.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0047-1860
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
87-92
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
[Interference in endotoxin and fungal polysaccharide assays from blood products and antimicrobial agents].
pubmed:affiliation
Department of Traumatology and Emergency Medicine, Osaka University Medical School.
pubmed:publicationType
Journal Article, English Abstract