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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-4-12
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pubmed:abstractText |
1 Neoglycoalbumin (NGA) was synthesised by covalent coupling of 2-imino-2-methoxyethyl-1-thio-beta-D-galactopyranoside (IME-thiogalactose) to the primary amino groups of human serum albumin (HSA). NGA was purified by ultrafiltration and size exclusion h.p.l.c. (SEC). 99mTc-labelling was performed with and without SEC purification. 2 Estimation of 99mTc-NGA-binding to human hepatic binding protein (HBP) revealed a complex behaviour indicating saturable high- and low-affinity sites. The high-affinity binding capacity was 1.1 +/- 0.4 pmol mg-1 human liver plasma membrane protein, the low-affinity binding capacity was 6.2 +/- 1.8 pmol mg-1 liver plasma membrane protein. The apparent equilibrium dissociation constants were 2.4 +/- 1.2 and 18.4 +/- 4.8 nM, respectively. 3 Specific binding of 99mTc-NGA to human HBP in the presence of 100 microM unlabelled NGA, Ca++ and Mg++ at pH 7.5 and 37 degrees C reached 85 +/- 5% at equilibrium. The amount of ligand specifically bound increased with the amount of human liver membrane protein added. The concentration of unlabelled agonist necessary to displace 50% of ligand bound amounted to 100 nM.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-1254568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-13691482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-2464364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-2469102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-2997417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-4045560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-4370480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-4402937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-4967807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-5916390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-6048188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-6244303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-6413351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-6701314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-6737077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-6777603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-6947268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-7250640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-7372599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-7375938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-7407914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-7430081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-7438047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2306412-762112
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0306-5251
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
207-14
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1990
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pubmed:articleTitle |
99mTc-neoglycoalbumin (NGA)-binding to human hepatic binding protein (HBP) in vitro.
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pubmed:affiliation |
Second Department of Nuclear Medicine, University of Vienna, Austria.
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pubmed:publicationType |
Journal Article,
In Vitro
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