Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2 Pt 1
|
| pubmed:dateCreated |
1990-3-28
|
| pubmed:abstractText |
To assess the roles of acid-base transport systems in cell volume regulation in rat hepatocytes, intracellular pH (pHi) was measured in subconfluent monolayers loaded with 2'-7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) after exposure to hypotonic and relative hypertonic media, interventions that stimulate regulatory volume decrease (RVD) and increase (RVI), respectively. During RVD, pHi decreased from 6.98 +/- 0.11 to 6.85 +/- 0.08 in the absence of HCO3- and from 7.26 +/- 0.10 to 7.19 +/- 0.06 in its presence. Omission of Na+ or addition of 1 mM amiloride prevented the decline in pHi. Acute withdrawal or replacement of Na+ in hypotonic medium resulted in a slower rate of fall or recovery in pHi, respectively, than when the same maneuvers were carried out in isotonic medium. In contrast, during RVI, pHi increased from 6.86 +/- 0.11 to 7.15 +/- 0.15 in the absence of HCO3-, a rise in pHi that was also completely abolished by Na+ removal or by 1 mM amiloride. In the presence of HCO3-, the rise in pHi was less marked than in its absence, although net acid efflux was greater because of a greater intracellular buffering capacity. Cl- removal in the presence of HCO3- had no effect on the change in pHi during either RVD or RVI. Perfusion with 0.5 mM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) during RVD lowered pHi further and accentuated the subsequent pHi rise seen after the return to isotonic medium. These data suggest that Na(+)-H+ exchange in rat hepatocytes is downregulated during RVD and activated during RVI. Cl(-)-HCO3- exchange does not appear to be involved in hepatocyte volume regulation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/4-Acetamido-4'-isothiocyanatostilben...,
http://linkedlifedata.com/resource/pubmed/chemical/Bicarbonates,
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Choline,
http://linkedlifedata.com/resource/pubmed/chemical/Hypertonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Hypotonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
258
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
G299-307
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2305896-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:2305896-4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid,
pubmed-meshheading:2305896-Animals,
pubmed-meshheading:2305896-Bicarbonates,
pubmed-meshheading:2305896-Buffers,
pubmed-meshheading:2305896-Cells, Cultured,
pubmed-meshheading:2305896-Choline,
pubmed-meshheading:2305896-Hydrogen-Ion Concentration,
pubmed-meshheading:2305896-Hypertonic Solutions,
pubmed-meshheading:2305896-Hypotonic Solutions,
pubmed-meshheading:2305896-Intracellular Fluid,
pubmed-meshheading:2305896-Kinetics,
pubmed-meshheading:2305896-Liver,
pubmed-meshheading:2305896-Male,
pubmed-meshheading:2305896-Osmolar Concentration,
pubmed-meshheading:2305896-Rats,
pubmed-meshheading:2305896-Rats, Inbred Strains,
pubmed-meshheading:2305896-Sodium
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Effects of osmotic stresses on isolated rat hepatocytes. II. Modulation of intracellular pH.
|
| pubmed:affiliation |
Department of Medicine and Liver Center, Yale University School of Medicine, New Haven, Connecticut 06510.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|