Linked Life Data

2302256

Source:http://linkedlifedata.com/resource/pubmed/id/2302256

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-3-1
pubmed:abstractText
The effect of the aromatic aldehydes benzaldehyde and salicylaldehyde, the glucose-acetal derivative 4,6-benzylidene-D-glucose (BG) and the glucoside salicylaldehyde-beta-D-glucoside (helicin) on cell inactivation induced by cis-dichlorodiammineplatinum (cis-DDP) was investigated using cultured human NHIK 3025 cells. Cell inactivation was measured as loss in the ability of single cells to give rise to macroscopic colonies following drug treatment. The fraction of cells surviving a 2 hr treatment with 10 microM cis-DDP increased from 0.012 +/- 0.004 to 0.10 +/- 0.03 when treatment was combined with at least 1 mM benzaldehyde or at least 0.2 mM salicylaldehyde. Of the two sugar-aldehyde derivatives only helicin protected cells from the inactivating effect of cis-DDP, although to a much lesser extent than either benzaldehyde or salicylaldehyde. While helicin retains the aldehyde moiety of salicylaldehyde, BG does not possess any free aldehyde group. Using synchronized cells we found these effects to appear in all phases of the cell cycle. Measurements of cell-associated platinum indicated that the degree of protection from the inactivating effects of cis-DDP by these aldehydes was related to the degree of reduced platinum accumulation. We conclude that this reduced accumulation may represent an inhibition of specific cell membrane uptake sites via Schiff based formation between membrane amino groups and aldehydes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-18
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Requirement of a reactive aldehyde moiety for aldehyde-mediated protection against cis-dichlorodiammineplatinum-induced cell inactivation.
pubmed:affiliation
Department of Tissue Culture, Norwegian Radium Hospital, Oslo.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't