| pubmed-article:2297705 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2297705 | lifeskim:mentions | umls-concept:C0006142 | lld:lifeskim |
| pubmed-article:2297705 | lifeskim:mentions | umls-concept:C1516213 | lld:lifeskim |
| pubmed-article:2297705 | lifeskim:mentions | umls-concept:C0042036 | lld:lifeskim |
| pubmed-article:2297705 | lifeskim:mentions | umls-concept:C0221920 | lld:lifeskim |
| pubmed-article:2297705 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:2297705 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:2297705 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2297705 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2297705 | pubmed:dateCreated | 1990-3-9 | lld:pubmed |
| pubmed-article:2297705 | pubmed:abstractText | A tumor-associated epidermal growth factor (EGF)-like activity was detected in the urine of breast cancer patients by means of an EGF radioreceptor assay and an anchorage-independent growth assay. The clonogenic growth factor activity of pooled void volume eluate fractions from a Bio-Gel P-30 column was completely neutralized by an anti-human epidermal growth factor antiserum but not by an anti-transforming growth factor alpha antiserum. This activity was determined in the urine of 71 breast cancer patients. A statistically significant correlation was found between EGF-like clonogenic activity and axillary lymph node status, tumor size, stage of disease, and grade of differentiation of the primary tumor. The Bio-Gel P-30 void volume fraction was used to purify the EGF-related polypeptide to apparent homogeneity by subsequent binding to and elution from A431 cells followed by isoelectric focusing. A polypeptide of a pI of approximately 3.4 was identified to be related to EGF by neutralization and immunoprecipitation experiments with anti-human epidermal growth factor antisera. This polypeptide migrated as a single band of Mr 43,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. | lld:pubmed |
| pubmed-article:2297705 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2297705 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2297705 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2297705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2297705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2297705 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2297705 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2297705 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:2297705 | pubmed:author | pubmed-author:FischerJJ | lld:pubmed |
| pubmed-article:2297705 | pubmed:author | pubmed-author:GrossiVV | lld:pubmed |
| pubmed-article:2297705 | pubmed:author | pubmed-author:EckertKK | lld:pubmed |
| pubmed-article:2297705 | pubmed:author | pubmed-author:NexøEE | lld:pubmed |
| pubmed-article:2297705 | pubmed:author | pubmed-author:GranetznyAA | lld:pubmed |
| pubmed-article:2297705 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2297705 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2297705 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:2297705 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2297705 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2297705 | pubmed:pagination | 642-7 | lld:pubmed |
| pubmed-article:2297705 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:meshHeading | pubmed-meshheading:2297705-... | lld:pubmed |
| pubmed-article:2297705 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2297705 | pubmed:articleTitle | A Mr 43,000 epidermal growth factor-related protein purified from the urine of breast cancer patients. | lld:pubmed |
| pubmed-article:2297705 | pubmed:affiliation | Central Institute of Molecular Biology, Academy of Sciences, German Democratic Republic, Berlin-Buch. | lld:pubmed |
| pubmed-article:2297705 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2297705 | lld:pubmed |
