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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1990-3-9
|
| pubmed:abstractText |
A tumor-associated epidermal growth factor (EGF)-like activity was detected in the urine of breast cancer patients by means of an EGF radioreceptor assay and an anchorage-independent growth assay. The clonogenic growth factor activity of pooled void volume eluate fractions from a Bio-Gel P-30 column was completely neutralized by an anti-human epidermal growth factor antiserum but not by an anti-transforming growth factor alpha antiserum. This activity was determined in the urine of 71 breast cancer patients. A statistically significant correlation was found between EGF-like clonogenic activity and axillary lymph node status, tumor size, stage of disease, and grade of differentiation of the primary tumor. The Bio-Gel P-30 void volume fraction was used to purify the EGF-related polypeptide to apparent homogeneity by subsequent binding to and elution from A431 cells followed by isoelectric focusing. A polypeptide of a pI of approximately 3.4 was identified to be related to EGF by neutralization and immunoprecipitation experiments with anti-human epidermal growth factor antisera. This polypeptide migrated as a single band of Mr 43,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
642-7
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2297705-Biological Assay,
pubmed-meshheading:2297705-Breast Neoplasms,
pubmed-meshheading:2297705-Chromatography, Gel,
pubmed-meshheading:2297705-Cross Reactions,
pubmed-meshheading:2297705-Epidermal Growth Factor,
pubmed-meshheading:2297705-Growth Substances,
pubmed-meshheading:2297705-Humans,
pubmed-meshheading:2297705-Isoelectric Point,
pubmed-meshheading:2297705-Molecular Weight,
pubmed-meshheading:2297705-Precipitin Tests
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
A Mr 43,000 epidermal growth factor-related protein purified from the urine of breast cancer patients.
|
| pubmed:affiliation |
Central Institute of Molecular Biology, Academy of Sciences, German Democratic Republic, Berlin-Buch.
|
| pubmed:publicationType |
Journal Article
|