|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0005715,
umls-concept:C0025252,
umls-concept:C0030705,
umls-concept:C0043393,
umls-concept:C0204727,
umls-concept:C0205314,
umls-concept:C0205409,
umls-concept:C0679622,
umls-concept:C1998793,
umls-concept:C2347885
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1990-2-14
|
|
pubmed:abstractText |
No well-defined Blastomyces-specific antigens are currently available. We used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting to identify immunologically active molecules in the cell wall of B. dermatitidis. A major immunoreactive 120-kD protein (WI-1) was present in all five strains studied and comprised 5% of the protein in the cell wall extract obtained after freezing and thawing yeast cells. WI-1 was recognized by serum from all 10 patients with blastomycosis but by none of those from 5 patients with histoplasmosis. It was purified by electroelution, radiolabeled with 125I, and incorporated into a radioimmunoassay (RIA) for serodiagnosis of blastomycosis. Antibody to WI-1 was detected in 58 (85%) of 68 patients with blastomycosis (geometric mean titer, 1:2,981), in two (3%) of 73 patients with histoplasmosis, coccidioidomycosis, sporotrichosis, or candidiasis (titers, 1:86 and 1:91) and in none of 44 healthy persons. WI-1 was shown to be a surface molecule abundant on B. dermatitidis yeasts that were indirectly stained with serum from a rabbit immunized with WI-1. Approximately 0.93 pg of WI-1 or 4.7 x 10(6) WI-1 molecules were found on the surface of an individual yeast using an antigen-inhibition RIA; none was found on Histoplasma capsulatum or Candida albicans yeasts. We conclude that WI-1 is a novel, immunologically active surface molecule on the invasive form of B. dermatitidis and that WI-1 can be used to reliably detect antibody and study the immunopathogenesis of blastomycosis.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-13273400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-13590840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-13721936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-15828186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-2422200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-3079974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-3100658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-3688635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-3945290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-405328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-4128880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-4135392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-4135463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-4279334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-4427610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-4811099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-5095293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-5326107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-5557599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-5776517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-6339652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-6790440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-7002793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-808868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2295693-965086
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0021-9738
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
85
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
152-61
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2295693-Antibodies, Fungal,
pubmed-meshheading:2295693-Antigens, Fungal,
pubmed-meshheading:2295693-Blastomyces,
pubmed-meshheading:2295693-Blastomycosis,
pubmed-meshheading:2295693-Blotting, Western,
pubmed-meshheading:2295693-Cell Wall,
pubmed-meshheading:2295693-Dermatomycoses,
pubmed-meshheading:2295693-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2295693-Humans,
pubmed-meshheading:2295693-Iodine Radioisotopes,
pubmed-meshheading:2295693-Isotope Labeling,
pubmed-meshheading:2295693-Lung Diseases,
pubmed-meshheading:2295693-Membrane Proteins,
pubmed-meshheading:2295693-Microscopy, Electron,
pubmed-meshheading:2295693-Microscopy, Electron, Scanning,
pubmed-meshheading:2295693-Molecular Weight,
pubmed-meshheading:2295693-Radioimmunoassay
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Isolation, purification, and radiolabeling of a novel 120-kD surface protein on Blastomyces dermatitidis yeasts to detect antibody in infected patients.
|
|
pubmed:affiliation |
Department of Pediatrics, University of Wisconsin Medical School, University of Wisconsin Hospital and Clinics 53792.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
