Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1990-2-15
|
| pubmed:abstractText |
To identify sequences necessary for lens-specific gene expression, lines of transgenic mice were generated which contain murine alpha A-crystallin promoter sequences [-111 to +46 (alpha 111), -88 to +46 (alpha 88), and -34 to +46 (alpha 34)] fused to the bacterial chloramphenicol acetyltransferase (CAT) gene and CAT expression was analyzed. Mice carrying the alpha 111-CAT or the alpha 88-CAT fusion transgene expressed CAT exclusively in lens, except for one line containing alpha 111-CAT, which expressed low levels of CAT in several nonlenticular tissues. Transcription from these promoters in lens initiated at the same site as the endogenous alpha A-crystallin promoter. In one line of mice alpha 88-CAT transgene became active in the lens during embryonic development at approximately the same time that the alpha A-crystallin gene normally begins to be expressed. In contrast, the alpha 34-CAT fusion transgene, containing the TATA box but no sequences further upstream, was inactive in transgenic mice. Our data suggest that 134 bp of sequence (-88 to +46) in the murine alpha A-crystallin gene is sufficient to provide lens specificity, although we cannot rule out the possibility that other sequences also contribute to promoter function.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0012-1606
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
137
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
68-76
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2295367-Animals,
pubmed-meshheading:2295367-Base Sequence,
pubmed-meshheading:2295367-Crystallins,
pubmed-meshheading:2295367-DNA,
pubmed-meshheading:2295367-Embryo, Mammalian,
pubmed-meshheading:2295367-Embryonic and Fetal Development,
pubmed-meshheading:2295367-Female,
pubmed-meshheading:2295367-Gene Expression,
pubmed-meshheading:2295367-Gene Expression Regulation,
pubmed-meshheading:2295367-Genes,
pubmed-meshheading:2295367-Lens, Crystalline,
pubmed-meshheading:2295367-Male,
pubmed-meshheading:2295367-Mice,
pubmed-meshheading:2295367-Mice, Inbred Strains,
pubmed-meshheading:2295367-Mice, Transgenic,
pubmed-meshheading:2295367-Molecular Sequence Data,
pubmed-meshheading:2295367-Oligonucleotide Probes,
pubmed-meshheading:2295367-Organ Specificity,
pubmed-meshheading:2295367-Polymerase Chain Reaction,
pubmed-meshheading:2295367-Promoter Regions, Genetic,
pubmed-meshheading:2295367-RNA, Messenger
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Regulation of the murine alpha A-crystallin promoter in transgenic mice.
|
| pubmed:affiliation |
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
|
| pubmed:publicationType |
Journal Article
|