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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-2-22
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pubmed:abstractText |
Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a).
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
1037
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
129-32
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2294968-Amino Acid Sequence,
pubmed-meshheading:2294968-Apolipoproteins B,
pubmed-meshheading:2294968-Cysteine,
pubmed-meshheading:2294968-Fluoresceins,
pubmed-meshheading:2294968-Humans,
pubmed-meshheading:2294968-Lipoproteins, LDL,
pubmed-meshheading:2294968-Male,
pubmed-meshheading:2294968-Molecular Sequence Data,
pubmed-meshheading:2294968-Peptide Fragments,
pubmed-meshheading:2294968-Spectrometry, Fluorescence,
pubmed-meshheading:2294968-Sulfhydryl Reagents
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pubmed:year |
1990
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pubmed:articleTitle |
Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine.
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pubmed:affiliation |
Department of Medicine, Baylor College of Medicine, Methodist Hospital, Houston, TX 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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