Linked Life Data

2291975

Source:http://linkedlifedata.com/resource/pubmed/id/2291975

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1991-4-9
pubmed:abstractText
We have utilized primary cultures of free-floating clusters obtained from pancreata of human cadaveric fetuses. Clusters have been incubated for 10 days in RPMI-1640 medium with 3H-thymidine and various concentrations of fetal calf serum and glucose. Viability of clusters was assessed by insulin radioimmunoassay. Cell reproduction was investigated by radioautography. We have revealed 2 populations of proliferating epithelial cells in clusters: the minor population of cells of duct-like structures and the major population of other epithelial cells. Rate of accumulation of labelled cells in both populations in clusters of 17-18-week-old fetuses was significantly higher than in clusters of 23-24-week-old fetuses. Proliferation of cells of duct-like structures was regulated by serum but not by glucose, whereas proliferation of other epithelial cells depended on both serum and glucose concentrations.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0365-9615
pubmed:author
pubmed:issnType
Print
pubmed:volume
110
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
206-8
pubmed:dateRevised
2008-10-8
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
[Effects of serum and glucose on the proliferation of different types of cells of the human embryonic pancreas in primary culture].
pubmed:publicationType
Journal Article, English Abstract