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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1991-3-29
|
| pubmed:abstractText |
Term cytotrophoblast do not express polymorphic MHC Class I antigens, unlike other fetal and maternal cells in the amniochorion/decidua. This allows cytotrophoblast to be isolated and purified from this tissue, utilizing 4E, a monoclonal antibody specific for HLA-B, which labels only non-trophoblast. We have developed a method using enzymic dispersion and Percoll gradient centrifugation, followed by flow cytometry, that yields, on average, a total of 5 X 10(6) term extravillous cytotrophoblast, 97 per cent pure. The availability of highly purified extravillous cytotrophoblast, for the first time, permits precise investigation of trophoblast function.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0143-4004
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
11
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
505-13
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2290802-Antibodies, Monoclonal,
pubmed-meshheading:2290802-Cell Separation,
pubmed-meshheading:2290802-Centrifugation, Density Gradient,
pubmed-meshheading:2290802-Female,
pubmed-meshheading:2290802-Flow Cytometry,
pubmed-meshheading:2290802-Humans,
pubmed-meshheading:2290802-Immunohistochemistry,
pubmed-meshheading:2290802-Placenta,
pubmed-meshheading:2290802-Trophoblasts
|
| pubmed:articleTitle |
Purification of human cytotrophoblast from term amniochorion by flow cytometry.
|
| pubmed:affiliation |
Harris-Birthright Pre-eclampsia Unit, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Maternity Department, Headington, Oxford, UK.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|