Linked Life Data

2288875

Source:http://linkedlifedata.com/resource/pubmed/id/2288875

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1991-4-4
pubmed:abstractText
The ras protein can be viewed as molecular switches for undetermined signal transduction pathways. We are interested in defining the downstream effectors and targets associated with ras signal transduction. A nuclear target of ras action is the conserved sequence element TGACTCT, which functions as a ras-responsive transcriptional element (RRE). An Mr 120,000 nuclear factor present in transformed murine cells recognizes the RRE. Mutation of the conserved RRE element to the palindromic element AGACTCT created a binding site that is recognized with 5-fold greater affinity by the Mr 120,000 factor. The palindromic element functions as an RRE as determined by transient transfection assays. UV-crosslinking of nuclear factors in human tumor cell lines to the palindromic element revealed that an Mr 120,000 factor that recognized RREs was present in cells that contain activated Ha- or N-ras genes, but not in human tumor cells that lack activated ras. Expression of exogenous activated ras in a human tumor cell line that lacks the oncogene induced the Mr 120,000 factor. The Mr 120,000 factor, which we have termed ras-responsive factor 1, is an intermediate in the signal transduction pathway that links ras to the nucleus and may play a role in the initiation or progression of human tumors containing an activated ras gene.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1044-9523
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:geneSymbol
ras
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
601-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
A nuclear factor that binds to ras-responsive enhancer elements is present in human tumor cells.
pubmed:affiliation
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't