Linked Life Data

2282643

Source:http://linkedlifedata.com/resource/pubmed/id/2282643

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-3-19
pubmed:abstractText
The three-dimensional architecture of the cytoskeleton in cultured fibroblasts was studied using a newly devised technique for revealing cell interiors and an ultrahigh resolution scanning electron microscope, the UHS-T1. Both the cytoskeleton and membranous structures such as the plasma membrane and endoplasmic reticulum were well preserved, and we could observe the relationship between both components. Actin filaments, intermediate filaments, and microtubules were identified by immunogold staining with 5-15 nm gold particles. Actin filaments, measuring 10 nm in diameter in material not metal coated, formed thick bundles (stress fibers), sheaths or meshworks. Just beneath the plasma membrane, actin filaments could be seen in a two-dimensional network, with fibers linked laterally to the membrane. Intermediate filaments, 12 nm in diameter in uncoated material, were observed mainly in the perikaryon. Microtubules (26 nm) and clathrin-coated vesicles were also clearly seen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1122-9497
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
591-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
A preparation technique for observing cytoskeletons by high resolution scanning electron microscopy.
pubmed:affiliation
Department of Anatomy, Tottori University School of Medicine, Yonago, Japan.
pubmed:publicationType
Journal Article